APC Anti-Mouse CD3 Antibody[17A2]

货号：E-AB-F1013UE
文献（6）
说明书

说明书

Price: ¥900 ¥360

Size:

100μg 25μg

应用性： FCM
亚型： Rat IgG2b, κ
宿主： Rat

Background	CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.
Alternate Names	T-cell surface glycoprotein CD 3epsilon/delta/gamma/zeta chain;CD3E/D/G/Z;CD3e/d/g/z;CD3E/D/G/Z;CD3;
Swissprot	P04235;P11942;P22646;P24161;
Gene ID	12502
Clone No	17A2
Host	Rat
Reactivity	Mouse
Application	FCM
Isotype	Rat IgG2b, κ
Isotype Control	APC Rat IgG2b, κ Isotype Control[LTF-2]
Conjugation	APC
Conjugation Information	APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter).
Spectrum
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
Storage	This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
Expiration date	12个月
Shipping	冰袋

C57BL/6 murine splenocytes are stained with APC Anti-Mouse CD3 Antibody (filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.

Self-Activated Cascade Biocatalysis of Glucose Oxidase–Polycation–Iron Nanoconjugates Augments Cancer Immunotherapy

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DOI：10.1021/acsami.2c04894
The petroleum ether extract of Brassica rapa L. induces apoptosis of lung adenocarcinoma cells via the mitochondria-dependent pathway

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Journal：Food & Function

DOI：10.1039/D1FO01547H

PMID：34523644
Immunomodulatory effects and mechanisms of Tiepishihu Xiyangshen granules on cyclophosphamide induced immuno-suppression via TLR4/MAPKs and PI3K/AKT/FOXO3a signal pathways

IF: 5.195

Journal：JOURNAL OF ETHNOPHARMACOLOGY

DOI：10.1016/j.jep.2023.116192
In Vitro and In Vivo Dendritic Cell Immune Stimulation Effect of Low Molecular Weight Fucoidan from New Zealand Undaria pinnatifida

IF: 5.118

Journal：Marine Drugs

DOI：10.3390/md20030197

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Changes of macrophage and CD4+ T cell in inflammatory response in type 1 diabetic mice

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DOI：10.1038/s41598-022-19031-9

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Induction of Th1 and Th2 in the protection against SARS-CoV-2 through mucosal delivery of an adenovirus vaccine expressing an engineered spike protein

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Journal：VACCINE

DOI：10.1016/j.vaccine.2021.12.024

PMID：34952759

E-AB-F10130	AF/LE Purified Anti-Mouse CD3 Antibody[17A2]	500μg , 50μg , 1mg
E-AB-F1013A	Purified Anti-Mouse CD3 Antibody[17A2]	100μg , 25μg
E-AB-F1013B	Biotin Anti-Mouse CD3 Antibody[17A2]	100μg , 25μg
E-AB-F1013C	FITC Anti-Mouse CD3 Antibody[17A2]	100Tests , 50Tests , 100Tests×2
E-AB-F1013D	PE Anti-Mouse CD3 Antibody[17A2]	100Tests , 50Tests , 100Tests×2
E-AB-F1013E	APC Anti-Mouse CD3 Antibody[17A2]	100Tests , 50Tests , 100Tests×2
E-AB-F1013F	PerCP Anti-Mouse CD3 Antibody[17A2]	100Tests , 50Tests , 100Tests×2
E-AB-F1013G	PE/Cyanine5 Anti-Mouse CD3 Antibody[17A2]	100Tests , 50Tests , 100Tests×2
E-AB-F1013H	PE/Cyanine7 Anti-Mouse CD3 Antibody[17A2]	100Tests , 50Tests , 100Tests×2
E-AB-F1013I	PE/Cyanine5.5 Anti-Mouse CD3 Antibody[17A2]	100Tests , 50Tests , 100Tests×2
E-AB-F1013J	PerCP/Cyanine5.5 Anti-Mouse CD3 Antibody[17A2]	100Tests , 50Tests , 100Tests×2
E-AB-F1013L	Elab Fluor® 488 Anti-Mouse CD3 Antibody[17A2]	100Tests , 50Tests , 100Tests×2
E-AB-F1013M	Elab Fluor® 647 Anti-Mouse CD3 Antibody[17A2]	100Tests , 50Tests , 100Tests×2
E-AB-F1013P	PE/Elab Fluor® 594 Anti-Mouse CD3 Antibody[17A2]	100Tests , 50Tests , 100Tests×2
E-AB-F1013Q	Elab Fluor® Violet 450 Anti-Mouse CD3 Antibody[17A2]	100Tests , 50Tests , 100Tests×2
E-AB-F1013S	Elab Fluor® Red 780 Anti-Mouse CD3 Antibody[17A2]	100Tests , 50Tests , 100Tests×2
E-AB-F1013UC	FITC Anti-Mouse CD3 Antibody[17A2]	100μg , 25μg
E-AB-F1013UD	PE Anti-Mouse CD3 Antibody[17A2]	100μg , 25μg
E-AB-F1013UF	PerCP Anti-Mouse CD3 Antibody[17A2]	100μg , 25μg
E-AB-F1013UG	PE/Cyanine5 Anti-Mouse CD3 Antibody[17A2]	100μg , 25μg
E-AB-F1013UH	PE/Cyanine7 Anti-Mouse CD3 Antibody[17A2]	100μg , 25μg
E-AB-F1013UI	PE/Cyanine5.5 Anti-Mouse CD3 Antibody[17A2]	100μg , 25μg
E-AB-F1013UJ	PerCP/Cyanine5.5 Anti-Mouse CD3 Antibody[17A2]	100μg , 25μg
E-AB-F1013UL	Elab Fluor® 488 Anti-Mouse CD3 Antibody[17A2]	100μg , 25μg
E-AB-F1013UM	Elab Fluor® 647 Anti-Mouse CD3 Antibody[17A2]	100μg , 25μg
E-AB-F1013UP	PE/Elab Fluor® 594 Anti-Mouse CD3 Antibody[17A2]	100μg , 25μg
E-AB-F1013UQ	Elab Fluor® Violet 450 Anti-Mouse CD3 Antibody[17A2]	100μg , 25μg
E-AB-F1013US	Elab Fluor® Red 780 Anti-Mouse CD3 Antibody[17A2]	100μg , 25μg

Q1:流式抗体与免疫组化抗体有什么区别？

流式抗体是经过流式实验验证的，一般是针对单一种属的直接带荧光标记的单克隆抗体，检测的是单细胞悬液，结果通过流式细胞仪分析。免疫组化抗体是经过免疫组化实验验证的单克隆或者多克隆抗体，抗体通常情况下不带标记，通过酶标二抗催化底物显色，结果通过普通光学显微镜观察。

Q2:流式抗体与二抗有什么区别？

流式抗体是经过流式实验验证的，一般是针对单一种属的直接带荧光标记的单克隆抗体，检测的是单细胞悬液，结果通过流式细胞仪分析。二抗一般不能直接识别到抗原，需要通过结合能识别目标蛋白的一抗间接偶联到目标蛋白上。针对不同的实验应用类型，二抗上面可以选择不同的标记，如做WB和IHC实验，通常情况下选择带HRP酶的二抗，做IF实验，一般选择带FITC、CY3等荧光基团的二抗，也有些二抗标记biotin， biotin自身并不带荧光集团，也不能催化底物显色或者发出荧光，因此选择biotin标记的二抗，还需要选择HRP酶或者荧光标记的链霉亲和素偶联到生物素上，进一步反应才能检测到目标蛋白。

Q3:流式抗体都要同型对照吗？

一般建议所有流式实验中都使用同型对照抗体作为阴阳性细胞的判断依据，尤其是低表达或连续表达的指标，数据分析的时候不好圈门，同型对照抗体是必不可少的对照，但是对于一些分群明显的指标，如CD45、CD3、CD4、CD8等，可以不用做同型对照。

Q4:流式抗体和封闭抗体有什么区别？

流式抗体是经过流式实验验证的，一般是针对单一种属的直接带荧光标记的单克隆抗体，检测的是单细胞悬液，结果通过流式细胞仪分析。封闭抗体是流式实验中用于特异性封闭细胞表面FC受体的，不带荧光标记的纯抗，如检测小鼠的细胞样本，封闭抗体为Purified Anti-Mouse CD16/32 Monoclonal Antibody。封闭抗体的作用是减少流式抗体染色过程中的非特异性染色，如果需检测的是某些表面表达FC受体较丰富的细胞，如巨噬细胞，染色前必须使用封闭抗体。

Q5:怎样选流式抗体对应的同型对照？

同型对照是与染色的单克隆抗体： ①相同种属来源 ②相同免疫球蛋白及亚型 ③相同荧光素标记 ④相同剂量和浓度 ⑤由未免疫动物血清纯化而来用此消除由于抗体非特异性结合到细胞表面Fc受体而产生的背景染色。我们在官网上有详细说明，可以查看：https://www.elabscience.cn/List-detail-4813.html

Q6:流式抗体与免疫荧光抗体区别？

流式抗体是经过流式实验验证的，一般是针对单一种属的直接带荧光标记的单克隆抗体，检测的是单细胞悬液，结果通过流式细胞仪分析。
免疫荧光抗体是经过免疫荧光实验验证的单克隆或者多克隆抗体，抗体通常情况下一般不带标记，通过FITC、Cyanine 3等荧光标记二抗显色，结果通过荧光显微镜观察。

Q7:流式抗体与荧光标记抗体有什么区别？

流式抗体是经过流式实验验证的，一般是针对单一种属的直接带荧光标记的单克隆抗体，检测的是单细胞悬液，结果通过流式细胞仪分析。荧光标记抗体一般指的是带FITC、CY3等荧光标记的二抗，用于免疫荧光实验中识别对应的一抗，或者FITC、CY3标记的荧光一抗，可用于流式或免疫荧光实验，只有经过流式验证的荧光抗体才能称为流式抗体。

Q8:流式抗体和普通抗体有什么区别？

流式抗体是经过流式实验验证的，一般是针对单一种属的直接带荧光标记的单克隆抗体，检测的是单细胞悬液，结果通过流式细胞仪分析。普通抗体一般指不带标记的单克隆或者多克隆抗体，反应性和应用一般较广，能做多个种属的多种实验应用，最后实验结果的观察需要依赖酶催化底物显色或者发出荧光后检测，或者FITC、CY3等荧光标记的二抗发出荧光检测。

Q9:流式实验为什么要选同型对照？

同型对照抗体用于消除由于抗体非特异性结合到细胞表面Fc受体等而产生的背景染色，能够作为阴阳性细胞的判断依据，尤其是低表达或连续表达的指标，数据分析的时候不好圈门，同型对照抗体是必不可少的对照。