One-step TUNEL In Situ Apoptosis Kit (Green, FITC)
中文名称:一步法TUNEL原位细胞凋亡检测试剂盒(绿色,FITC)
Price:
Size:
100Assays
50Assays
20Assays
- 应用: 细胞凋亡-DNA片段化
检测原理 | 细胞在发生凋亡时,会激活一些特异性的DNA内切酶,这些内切酶会切断核小体间的基因组DNA。使得凋亡细胞的DNA被切割成180~200bp片段,在琼脂糖凝胶上通常以180~200bp的阶梯状迁移。TdT酶(脱氧核糖核酸末端转移酶)将标记的dUTP连接到断裂DNA暴露的3'-OH末端,通过在这些末端添加荧光dUTP的方式来标记晚期凋亡细胞,从而可以通过荧光显微镜进行检测。 |
检测方法 | 荧光法 |
样本类型 | 石蜡切片;冰冻切片;细胞爬片;细胞涂片 |
检测时间 | 3 hours |
检测仪器 | 荧光显微镜 |
荧光 | FITC |
Ex/Em | 490/520 |
Channel set | FITC |
Filter set | FITC |
自备试剂 | 4%多聚甲醛(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-BC-R187),ddH2O,含抗荧光淬灭剂的封片液(E-IR-R119),二甲苯,无水乙醇 |
保存温度 | 该产品可在-20°C避光条件下保存12个月。 |
有效期 | 12个月 |
运输条件 | 冰袋 |
Paraffin embedded rat heart was treated with DNase I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNase I treated sample (green). The cells were counterstained with DAPI (blue).This photo was taken by confocal microscope.
Paraffin embedded mouse liver was treated with DNase I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNase I treated sample (green). The cells were counterstained with DAPI (blue).This photo was taken by confocal microscope
Paraffin embedded mouse spleen was treated with DNase I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNase I treated sample (green). The cells were counterstained with DAPI (blue).This photo was taken by confocal microscop
Fluorescence microscope analysis of camptothecin-induced apoptosis of Hela cells.
Mixed samples of normal mouse spleen cells and DNase I treated mouse spleen cells were stained.
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