PE/Cyanine7 Anti-Mouse CD86 Antibody[GL-1]
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- 应用性: FCM
- 亚型: Rat IgG2a, κ
- 宿主: Rat
Background | CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is a ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells. |
Alternate Names | Activation B7-2 antigen;Cd86;ETC-1;Early T-cell costimulatory molecule 1;T-lymphocyte activation antigen CD86 |
Swissprot | |
Gene ID | |
Clone No | |
Host | Rat |
Reactivity | Mouse |
Application | |
Isotype | Rat IgG2a, κ |
Isotype Control | |
Conjugation | PE/Cyanine 7 |
Conjugation Information | PE/Cyanine7 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 775 nm (e.g., a 780/60 nm bandpass filter). |
Spectrum | |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA. |
Storage | This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze. |
Expiration date | 12个月 |
Shipping |
冰袋 |
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Q1:流式细胞仪分选Raw264.7 M1型和M2型细胞的标志物是什么?分选之后还能培养吗?
大多数做小鼠巨噬细胞分选会用F4/80、CD11b确定总巨噬细胞,然后用CD86测M1型,CD206测M2型,以上只作为一个参考。如果流式分选得到的细胞还需进行培养,建议不要使用CD206,因为CD206需要进行固定破膜操作,细胞会全部死亡。此外,M1和M2的标志物表达量不高,很难分选。
Q2:流式检测小鼠巨噬细胞,要检测M1、M2型巨噬细胞,有哪些推荐?
大多数老师做小鼠巨噬细胞会用F4/80、CD11b确定总巨噬细胞,然后用CD86测M1型,CD206测M2型,但是最终客户要检测哪些指标还是需要客户根据文献以及自己的实验需求自己来决定的,以上可以作为一个参考。